imaging software package cellprofiler Search Results


99
Oxford Instruments cellprofiler 75 76 imaris viewer
Cellprofiler 75 76 Imaris Viewer, supplied by Oxford Instruments, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cellprofiler 75 76 imaris viewer/product/Oxford Instruments
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cellprofiler 75 76 imaris viewer - by Bioz Stars, 2026-04
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Broad Institute Inc cellprofiler image analysis software
Cellprofiler Image Analysis Software, supplied by Broad Institute Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cellprofiler image analysis software/product/Broad Institute Inc
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cellprofiler image analysis software - by Bioz Stars, 2026-04
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Danaher Inc in cell analyzer 2000
In Cell Analyzer 2000, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/in cell analyzer 2000/product/Danaher Inc
Average 90 stars, based on 1 article reviews
in cell analyzer 2000 - by Bioz Stars, 2026-04
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Broad Institute Inc automated image quantification program cellprofiler 2.0
Automated Image Quantification Program Cellprofiler 2.0, supplied by Broad Institute Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/automated image quantification program cellprofiler 2.0/product/Broad Institute Inc
Average 90 stars, based on 1 article reviews
automated image quantification program cellprofiler 2.0 - by Bioz Stars, 2026-04
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Broad Institute Inc digital image analysis software (cellprofiler v.4.2.1
Digital Image Analysis Software (Cellprofiler V.4.2.1, supplied by Broad Institute Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/digital image analysis software (cellprofiler v.4.2.1/product/Broad Institute Inc
Average 90 stars, based on 1 article reviews
digital image analysis software (cellprofiler v.4.2.1 - by Bioz Stars, 2026-04
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CellPro Inc image analysis software cellprofiler v. 4.2.1
Image Analysis Software Cellprofiler V. 4.2.1, supplied by CellPro Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/image analysis software cellprofiler v. 4.2.1/product/CellPro Inc
Average 90 stars, based on 1 article reviews
image analysis software cellprofiler v. 4.2.1 - by Bioz Stars, 2026-04
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90
MathWorks Inc image analysis software cellprofiler
In situ detection of eIF4F subcellular distribution. (A) Left panel: schematic view of the proximity ligation assay for the eIF4E-eIF4G complex. 4E: eIF4E, 4 G: eIF4G, 4 A: eIF4A, AAA: polyA tail. Right panel: example images of the eIF4E-eIF4G PLA assay and cell segmentation. The eIF4E-eIF4G complex was stained following the proximity ligation assay protocol, the cytoskeleton was stained by Phalloidin-Alexa 488, and the nucleus was stained with Hoechst 33342. (B) Polysome profile and eIF4E-eIF4G PLA assay of A375 cells upon treatment with PP242. Cells were treated with PP424 at 1 μM for 24 h, and then lysed by polysome hypotonic buffer or fixed with 4% PFA. (C) Polysome profile and eIF4E-eIF4G PLA assay of QBC989 cholangiocarcinoma cells upon nutrient deprivation. Cells were cultured with HBSS solution for nutrient starvation for 16 h, followed by hypotonic buffer lysis or 4% PFA fixation. (D-E) A375 cells expressing the ERK-KTR reporter gene were treated with 1 μM vemurafenib for 24 h and the eIF4E-eIF4G PLA assay was performed. Single cell quantification of ERK-KTR nuclear translocation (p-ERK1/2) and eIF4E-eIF4G spot count are plotted. (F) Pipeline of the eIF4E-eIF4G PLA image analysis. The eIF4E-eIF4G complex was stained following the proximity ligation assay protocol, the cytoskeleton was stained with Phalloidin-Alexa 488, and the nucleus was stained with Hoechst 33342. Cell images were then subjected to <t>Cellprofiler</t> 2.0 analysis to identify the nucleus and cytoplasm. The eIF4E-eIF4G spots were identified by using Cellprofiler module ‘IdentifySpots.m′ followed by correlation with each cell. The localization pattern of the eIF4E-eIF4G spots was calculated with Cellprofiler module ‘MeasureLocalizationOfSpots.m′. (G) Example images of the cell segmentation and spot localization analysis. (H) Summary of the spot features and cell features measured by Cellprofiler 2.0.
Image Analysis Software Cellprofiler, supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
image analysis software cellprofiler - by Bioz Stars, 2026-04
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Broad Institute Inc algorithms cellprofiler v.9777
In situ detection of eIF4F subcellular distribution. (A) Left panel: schematic view of the proximity ligation assay for the eIF4E-eIF4G complex. 4E: eIF4E, 4 G: eIF4G, 4 A: eIF4A, AAA: polyA tail. Right panel: example images of the eIF4E-eIF4G PLA assay and cell segmentation. The eIF4E-eIF4G complex was stained following the proximity ligation assay protocol, the cytoskeleton was stained by Phalloidin-Alexa 488, and the nucleus was stained with Hoechst 33342. (B) Polysome profile and eIF4E-eIF4G PLA assay of A375 cells upon treatment with PP242. Cells were treated with PP424 at 1 μM for 24 h, and then lysed by polysome hypotonic buffer or fixed with 4% PFA. (C) Polysome profile and eIF4E-eIF4G PLA assay of QBC989 cholangiocarcinoma cells upon nutrient deprivation. Cells were cultured with HBSS solution for nutrient starvation for 16 h, followed by hypotonic buffer lysis or 4% PFA fixation. (D-E) A375 cells expressing the ERK-KTR reporter gene were treated with 1 μM vemurafenib for 24 h and the eIF4E-eIF4G PLA assay was performed. Single cell quantification of ERK-KTR nuclear translocation (p-ERK1/2) and eIF4E-eIF4G spot count are plotted. (F) Pipeline of the eIF4E-eIF4G PLA image analysis. The eIF4E-eIF4G complex was stained following the proximity ligation assay protocol, the cytoskeleton was stained with Phalloidin-Alexa 488, and the nucleus was stained with Hoechst 33342. Cell images were then subjected to <t>Cellprofiler</t> 2.0 analysis to identify the nucleus and cytoplasm. The eIF4E-eIF4G spots were identified by using Cellprofiler module ‘IdentifySpots.m′ followed by correlation with each cell. The localization pattern of the eIF4E-eIF4G spots was calculated with Cellprofiler module ‘MeasureLocalizationOfSpots.m′. (G) Example images of the cell segmentation and spot localization analysis. (H) Summary of the spot features and cell features measured by Cellprofiler 2.0.
Algorithms Cellprofiler V.9777, supplied by Broad Institute Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
algorithms cellprofiler v.9777 - by Bioz Stars, 2026-04
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90
Broad Institute Inc custom image-analysis pipeline in cellprofiler
In situ detection of eIF4F subcellular distribution. (A) Left panel: schematic view of the proximity ligation assay for the eIF4E-eIF4G complex. 4E: eIF4E, 4 G: eIF4G, 4 A: eIF4A, AAA: polyA tail. Right panel: example images of the eIF4E-eIF4G PLA assay and cell segmentation. The eIF4E-eIF4G complex was stained following the proximity ligation assay protocol, the cytoskeleton was stained by Phalloidin-Alexa 488, and the nucleus was stained with Hoechst 33342. (B) Polysome profile and eIF4E-eIF4G PLA assay of A375 cells upon treatment with PP242. Cells were treated with PP424 at 1 μM for 24 h, and then lysed by polysome hypotonic buffer or fixed with 4% PFA. (C) Polysome profile and eIF4E-eIF4G PLA assay of QBC989 cholangiocarcinoma cells upon nutrient deprivation. Cells were cultured with HBSS solution for nutrient starvation for 16 h, followed by hypotonic buffer lysis or 4% PFA fixation. (D-E) A375 cells expressing the ERK-KTR reporter gene were treated with 1 μM vemurafenib for 24 h and the eIF4E-eIF4G PLA assay was performed. Single cell quantification of ERK-KTR nuclear translocation (p-ERK1/2) and eIF4E-eIF4G spot count are plotted. (F) Pipeline of the eIF4E-eIF4G PLA image analysis. The eIF4E-eIF4G complex was stained following the proximity ligation assay protocol, the cytoskeleton was stained with Phalloidin-Alexa 488, and the nucleus was stained with Hoechst 33342. Cell images were then subjected to <t>Cellprofiler</t> 2.0 analysis to identify the nucleus and cytoplasm. The eIF4E-eIF4G spots were identified by using Cellprofiler module ‘IdentifySpots.m′ followed by correlation with each cell. The localization pattern of the eIF4E-eIF4G spots was calculated with Cellprofiler module ‘MeasureLocalizationOfSpots.m′. (G) Example images of the cell segmentation and spot localization analysis. (H) Summary of the spot features and cell features measured by Cellprofiler 2.0.
Custom Image Analysis Pipeline In Cellprofiler, supplied by Broad Institute Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/custom image-analysis pipeline in cellprofiler/product/Broad Institute Inc
Average 90 stars, based on 1 article reviews
custom image-analysis pipeline in cellprofiler - by Bioz Stars, 2026-04
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90
MathWorks Inc version r2021b
In situ detection of eIF4F subcellular distribution. (A) Left panel: schematic view of the proximity ligation assay for the eIF4E-eIF4G complex. 4E: eIF4E, 4 G: eIF4G, 4 A: eIF4A, AAA: polyA tail. Right panel: example images of the eIF4E-eIF4G PLA assay and cell segmentation. The eIF4E-eIF4G complex was stained following the proximity ligation assay protocol, the cytoskeleton was stained by Phalloidin-Alexa 488, and the nucleus was stained with Hoechst 33342. (B) Polysome profile and eIF4E-eIF4G PLA assay of A375 cells upon treatment with PP242. Cells were treated with PP424 at 1 μM for 24 h, and then lysed by polysome hypotonic buffer or fixed with 4% PFA. (C) Polysome profile and eIF4E-eIF4G PLA assay of QBC989 cholangiocarcinoma cells upon nutrient deprivation. Cells were cultured with HBSS solution for nutrient starvation for 16 h, followed by hypotonic buffer lysis or 4% PFA fixation. (D-E) A375 cells expressing the ERK-KTR reporter gene were treated with 1 μM vemurafenib for 24 h and the eIF4E-eIF4G PLA assay was performed. Single cell quantification of ERK-KTR nuclear translocation (p-ERK1/2) and eIF4E-eIF4G spot count are plotted. (F) Pipeline of the eIF4E-eIF4G PLA image analysis. The eIF4E-eIF4G complex was stained following the proximity ligation assay protocol, the cytoskeleton was stained with Phalloidin-Alexa 488, and the nucleus was stained with Hoechst 33342. Cell images were then subjected to <t>Cellprofiler</t> 2.0 analysis to identify the nucleus and cytoplasm. The eIF4E-eIF4G spots were identified by using Cellprofiler module ‘IdentifySpots.m′ followed by correlation with each cell. The localization pattern of the eIF4E-eIF4G spots was calculated with Cellprofiler module ‘MeasureLocalizationOfSpots.m′. (G) Example images of the cell segmentation and spot localization analysis. (H) Summary of the spot features and cell features measured by Cellprofiler 2.0.
Version R2021b, supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/version r2021b/product/MathWorks Inc
Average 90 stars, based on 1 article reviews
version r2021b - by Bioz Stars, 2026-04
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90
Carl Zeiss zeiss axioimager z2
In situ detection of eIF4F subcellular distribution. (A) Left panel: schematic view of the proximity ligation assay for the eIF4E-eIF4G complex. 4E: eIF4E, 4 G: eIF4G, 4 A: eIF4A, AAA: polyA tail. Right panel: example images of the eIF4E-eIF4G PLA assay and cell segmentation. The eIF4E-eIF4G complex was stained following the proximity ligation assay protocol, the cytoskeleton was stained by Phalloidin-Alexa 488, and the nucleus was stained with Hoechst 33342. (B) Polysome profile and eIF4E-eIF4G PLA assay of A375 cells upon treatment with PP242. Cells were treated with PP424 at 1 μM for 24 h, and then lysed by polysome hypotonic buffer or fixed with 4% PFA. (C) Polysome profile and eIF4E-eIF4G PLA assay of QBC989 cholangiocarcinoma cells upon nutrient deprivation. Cells were cultured with HBSS solution for nutrient starvation for 16 h, followed by hypotonic buffer lysis or 4% PFA fixation. (D-E) A375 cells expressing the ERK-KTR reporter gene were treated with 1 μM vemurafenib for 24 h and the eIF4E-eIF4G PLA assay was performed. Single cell quantification of ERK-KTR nuclear translocation (p-ERK1/2) and eIF4E-eIF4G spot count are plotted. (F) Pipeline of the eIF4E-eIF4G PLA image analysis. The eIF4E-eIF4G complex was stained following the proximity ligation assay protocol, the cytoskeleton was stained with Phalloidin-Alexa 488, and the nucleus was stained with Hoechst 33342. Cell images were then subjected to <t>Cellprofiler</t> 2.0 analysis to identify the nucleus and cytoplasm. The eIF4E-eIF4G spots were identified by using Cellprofiler module ‘IdentifySpots.m′ followed by correlation with each cell. The localization pattern of the eIF4E-eIF4G spots was calculated with Cellprofiler module ‘MeasureLocalizationOfSpots.m′. (G) Example images of the cell segmentation and spot localization analysis. (H) Summary of the spot features and cell features measured by Cellprofiler 2.0.
Zeiss Axioimager Z2, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
zeiss axioimager z2 - by Bioz Stars, 2026-04
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90
MathWorks Inc matab-based image analysis tool cellprofiler
In situ detection of eIF4F subcellular distribution. (A) Left panel: schematic view of the proximity ligation assay for the eIF4E-eIF4G complex. 4E: eIF4E, 4 G: eIF4G, 4 A: eIF4A, AAA: polyA tail. Right panel: example images of the eIF4E-eIF4G PLA assay and cell segmentation. The eIF4E-eIF4G complex was stained following the proximity ligation assay protocol, the cytoskeleton was stained by Phalloidin-Alexa 488, and the nucleus was stained with Hoechst 33342. (B) Polysome profile and eIF4E-eIF4G PLA assay of A375 cells upon treatment with PP242. Cells were treated with PP424 at 1 μM for 24 h, and then lysed by polysome hypotonic buffer or fixed with 4% PFA. (C) Polysome profile and eIF4E-eIF4G PLA assay of QBC989 cholangiocarcinoma cells upon nutrient deprivation. Cells were cultured with HBSS solution for nutrient starvation for 16 h, followed by hypotonic buffer lysis or 4% PFA fixation. (D-E) A375 cells expressing the ERK-KTR reporter gene were treated with 1 μM vemurafenib for 24 h and the eIF4E-eIF4G PLA assay was performed. Single cell quantification of ERK-KTR nuclear translocation (p-ERK1/2) and eIF4E-eIF4G spot count are plotted. (F) Pipeline of the eIF4E-eIF4G PLA image analysis. The eIF4E-eIF4G complex was stained following the proximity ligation assay protocol, the cytoskeleton was stained with Phalloidin-Alexa 488, and the nucleus was stained with Hoechst 33342. Cell images were then subjected to <t>Cellprofiler</t> 2.0 analysis to identify the nucleus and cytoplasm. The eIF4E-eIF4G spots were identified by using Cellprofiler module ‘IdentifySpots.m′ followed by correlation with each cell. The localization pattern of the eIF4E-eIF4G spots was calculated with Cellprofiler module ‘MeasureLocalizationOfSpots.m′. (G) Example images of the cell segmentation and spot localization analysis. (H) Summary of the spot features and cell features measured by Cellprofiler 2.0.
Matab Based Image Analysis Tool Cellprofiler, supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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matab-based image analysis tool cellprofiler - by Bioz Stars, 2026-04
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Image Search Results


In situ detection of eIF4F subcellular distribution. (A) Left panel: schematic view of the proximity ligation assay for the eIF4E-eIF4G complex. 4E: eIF4E, 4 G: eIF4G, 4 A: eIF4A, AAA: polyA tail. Right panel: example images of the eIF4E-eIF4G PLA assay and cell segmentation. The eIF4E-eIF4G complex was stained following the proximity ligation assay protocol, the cytoskeleton was stained by Phalloidin-Alexa 488, and the nucleus was stained with Hoechst 33342. (B) Polysome profile and eIF4E-eIF4G PLA assay of A375 cells upon treatment with PP242. Cells were treated with PP424 at 1 μM for 24 h, and then lysed by polysome hypotonic buffer or fixed with 4% PFA. (C) Polysome profile and eIF4E-eIF4G PLA assay of QBC989 cholangiocarcinoma cells upon nutrient deprivation. Cells were cultured with HBSS solution for nutrient starvation for 16 h, followed by hypotonic buffer lysis or 4% PFA fixation. (D-E) A375 cells expressing the ERK-KTR reporter gene were treated with 1 μM vemurafenib for 24 h and the eIF4E-eIF4G PLA assay was performed. Single cell quantification of ERK-KTR nuclear translocation (p-ERK1/2) and eIF4E-eIF4G spot count are plotted. (F) Pipeline of the eIF4E-eIF4G PLA image analysis. The eIF4E-eIF4G complex was stained following the proximity ligation assay protocol, the cytoskeleton was stained with Phalloidin-Alexa 488, and the nucleus was stained with Hoechst 33342. Cell images were then subjected to Cellprofiler 2.0 analysis to identify the nucleus and cytoplasm. The eIF4E-eIF4G spots were identified by using Cellprofiler module ‘IdentifySpots.m′ followed by correlation with each cell. The localization pattern of the eIF4E-eIF4G spots was calculated with Cellprofiler module ‘MeasureLocalizationOfSpots.m′. (G) Example images of the cell segmentation and spot localization analysis. (H) Summary of the spot features and cell features measured by Cellprofiler 2.0.

Journal: Computational and Structural Biotechnology Journal

Article Title: Spatial patterns of the cap-binding complex eIF4F in human melanoma cells

doi: 10.1016/j.csbj.2023.01.040

Figure Lengend Snippet: In situ detection of eIF4F subcellular distribution. (A) Left panel: schematic view of the proximity ligation assay for the eIF4E-eIF4G complex. 4E: eIF4E, 4 G: eIF4G, 4 A: eIF4A, AAA: polyA tail. Right panel: example images of the eIF4E-eIF4G PLA assay and cell segmentation. The eIF4E-eIF4G complex was stained following the proximity ligation assay protocol, the cytoskeleton was stained by Phalloidin-Alexa 488, and the nucleus was stained with Hoechst 33342. (B) Polysome profile and eIF4E-eIF4G PLA assay of A375 cells upon treatment with PP242. Cells were treated with PP424 at 1 μM for 24 h, and then lysed by polysome hypotonic buffer or fixed with 4% PFA. (C) Polysome profile and eIF4E-eIF4G PLA assay of QBC989 cholangiocarcinoma cells upon nutrient deprivation. Cells were cultured with HBSS solution for nutrient starvation for 16 h, followed by hypotonic buffer lysis or 4% PFA fixation. (D-E) A375 cells expressing the ERK-KTR reporter gene were treated with 1 μM vemurafenib for 24 h and the eIF4E-eIF4G PLA assay was performed. Single cell quantification of ERK-KTR nuclear translocation (p-ERK1/2) and eIF4E-eIF4G spot count are plotted. (F) Pipeline of the eIF4E-eIF4G PLA image analysis. The eIF4E-eIF4G complex was stained following the proximity ligation assay protocol, the cytoskeleton was stained with Phalloidin-Alexa 488, and the nucleus was stained with Hoechst 33342. Cell images were then subjected to Cellprofiler 2.0 analysis to identify the nucleus and cytoplasm. The eIF4E-eIF4G spots were identified by using Cellprofiler module ‘IdentifySpots.m′ followed by correlation with each cell. The localization pattern of the eIF4E-eIF4G spots was calculated with Cellprofiler module ‘MeasureLocalizationOfSpots.m′. (G) Example images of the cell segmentation and spot localization analysis. (H) Summary of the spot features and cell features measured by Cellprofiler 2.0.

Article Snippet: All the images were analyzed with the image analysis software CellProfiler , based on MATLAB_R2011a version (7.12.0.635 version, 64-bit).

Techniques: In Situ, Proximity Ligation Assay, Staining, Cell Culture, Lysis, Expressing, Translocation Assay